UJI BIOKIMIA KERENTANAN ANOPHELES ACONITUS TERHADAP INSEKTISIDA ORGANOFOSFAT (FENITROTHION) DAN KARBAMAT (BENDIOCARB) DI KABUPATEN JEPARA
Mardihusodo, Sugeng Juwono
Boewono, Damar Tri
MetadataShow full item record
AbstractOrganophosphate insecticide (fenitrothion) has been used in the vector control program on An. aconitus, the malaria vector, in Jepara Regency. However carbamate insecticides are recommended and used in many An. aconitus breedingsites (ricefield) for agricultural purposes. A large scale and continued applications of both insecticides can not control the target, because of various reasons such as reduced susceptibility or the development of vector resistance. The objective of this study are (1) to determine the susceptibility status of An. aconitus larvae to organophosphate (fenitrothion) and (2) to investigate the presence of two biochemical resistance mechanisms, possibly related to elevated esterase (non-specific esterase) and insensitive acetylcholinesterase. This research is a quasi-experimental with the Post-test only Control Group Design, wich was done at 9 districts of Jepara Regency. The methodes used were biochemical assays (microplate assays) for elevated esterase and insensitive acethylcholinesterase in mosquto larvae collected from the field, as well as those from Vector and Reservoir Control Research Unit (VRCRU) laboratory colony (control area). The biochemical test were respectively cross cheked with bioassays. The results were analyzed with One Way ANOVA and indicated that there was a statistically significant difference between the susceptibility status of An. aconitus mosquito larvae collected from the study area (Jepara Regency) and the control area (VRCRU colony). It can be concluded that An. aconitus mosquito larvae collected from the fields in Jepara Regency has shown reduced susceptibility to insecticides (fenitrothion) due to the elevated esterase rather than insensitive acetylcholinesterase.
- Repositori Balitbangkes