Abstract

An investigation was conducted on Bacillus thuringiensis H-14 local strain grown in coconut water media and Tryptose Phosphate Broth (TPB). The two medias were tested against Anopheles aconitus and Culex p. quinquefasciatus mosquito larvae in the Vector Control Research Station laboratory and in Sentinel traps village Kauman Kidul pool, Salatiga. The pathogenicity test on B. thuringiensis H-14 local strain grown in coconut water and TPB against An. aconitus and Cx. p. quinquefasciatus larvae in the laboratory was done according to WHO procedure. This was conducted to determine the LC50 and LC90 which is counted according to the probit analysis. The pathogenicity test result of B. thuringiensis H-14 local strain cultured in coconut water against An. aconitus and Cx. p. quinquefasciatus larvae at 24 hours of exposure, showed the LC50 were 0,04 ml/100ml and 0,10 ml/100ml with LC90 were 0,15 ml/100ml and 0,20 ml/100ml respectively. The pathogenicity test at 48 hours of exposure showed the LC50 were 0,01 ml/100ml and 0,02 ml/100ml with LC90 were 0,04 ml/100 ml and 0,13 ml/ 100 ml respectively. The pathogenicity test result ofB. thuringiensis H-14 local strain cultured in TP B media against An. aconitus and Cx. p. quinquefasciatus larvae at 24 hours exposure, showed the LC50 were 0,05 ml/100 ml and 0,06 ml/100 ml with LC90 were 0,20 ml/100 ml and 0,15 ml/100 ml respectively. The pathogenicity test at 48 hours of exposure showed the LC50 were 0,02 ml/100 ml and 0,03 ml/100ml with LC90 were 0,10 ml/100 ml and 0,08 ml/100ml respectively. The field trial showed the patogenicity test result of B. thuringiensis H-14 local strain cultured in coconut water media against An. aconitus larvae at Sentinel trap in rice field pools, showed a mortality of more than 50% for 4 days at the 0,15 ml/100 ml (LC90) aplication dosage. The pathogenicity against Cx. p. quinquefasciatus larvae at S.entinel trap was more than 50% for 3 days at the 0,20 ml/l100 ml (LC90) aplication dosage. However B. thuringiensis H-14 cultured in TPB against An. aconitus larvae at a dosage 0,20 ml/l00 ml and against Cx. p. quinquefasciatus larvae at a dosage of 0,15 ml/l00 ml showed a mortality of more than 50% for 3 days. The coconut water is suitable as a local mediafor culturing B. thuringiensis H-14.