Anemia in pregnancy adversely affected both to mother and fetus, in pregnancy, childbirth, postpartum and subsequent periods. Several factors causing anemia in pregnancy were pregnancies distance, maternal age and mother’s nutritional status (LILA). The aim of this study was to determine the relationship between pregnancy distance, maternal age, nutritional status (LILA) against anemia in pregnancy. Analytic observational with cross sectional approach was the type of this research. The sampling method was a simple random sampling. Sample of 120 people was obtained from medical records. The results of this study were tested by chi square test with significance (α = 0.05). Based on the chi-square test showed that there was a relationship between the distance pregnancy with anemia among pregnant women the first trimester with a p-value of 0.002, and there was no relationship between maternal age with anemia among pregnant women in the first trimester with a p-value of 0.241 and there was no relationship between nutritional status (LILA) with anemia among pregnant women in the first trimester with a p-value of 1.000. In conclution, there is a significant relationship between the distance in pregnancy and anemia among pregnant women in their first trimester. It is recommended for every mother to know the importance of interlude between pregnancies and limiting births.