A pregnancy check is one form of behavior. Which is influenced by factors related to behavior that is predisposing factor (age, sex, occupation, education, race, knowledge, attitude, belief, tradition and value), enabling factor. The program conducted by PuskesmasSragi 1 with policy of Puskesmas head to improve ANC program among others with Posyandu activity in each post for pregnant examination, pregnant mother's visit, procurement of pregnant mother's class regularly in village hall.The purpose of this study was to determine the study of descriptive level of knowledge about ANC with family support in third trimester pregnant women in Puskesmas Treatment Sragi 1 District SragiPekalongan Regency. This research type is descriptive with research that aims to describe the level of knowledge about ANC and family support in third trimester pregnant women in May - June 2017. Sampling technique that is with total sampling counted 40 people. Univariate analysis with frequency distributionThe results showed that the characteristic outcomes were aged between 20-35 years old as many as 37 people (92,5%), most of them did not work as many as 22 people(55%), mostly middle-level respondents education as many as 18 people (45%), parity in most multigravida as many as 26 people (65%). The level of knowledge of pregnant women about the visit of ANC in Puskesmas Care Sragi 1 District Sragi Pekalongan Regency mostly good as much 29 people (72,5%). Maternal family's support of ANC at PuskesmasSragi care 1 Sragi Sub-district Pekalongan Regency with value mostly supports 30 people (75%).