In this study, innovation of banana pseudostem nest was conducted to assess the physiological response of premature infants due to the provision of pseudostem banana nest innovation in the incubator. This innovation is needed because premature babies need conditions that promote physiological response functions to increase body temperature, gain weight and assess changes in oxygen saturation. Unfortunately, so far there is no temperature stabilizer in the incubator, the incubator is made of electric and heating elements only so that some physical parameters cannot be accommodated with the incubator. Therefore, this research proposes the innovation of pseudostem banana nest because it has a 400% water absorption level, a higher temperature stabilization on the fibers in commonly used materials. This research uses a quasi-experimental design method with pre-post test control group design. The sampling technique used was nonprobability sampling by consecutive sampling. A sample of 30 preterm infants was divided into 15 control group samples, 15 intervention group samples. Measurements were made on parameters of body temperature, weight, oxygen saturation before and after intervention in the control group and intervention group for three consecutive days. The results of this study were obtained significant differences in body temperature, oxygen saturation, incubator temperature before and after given nest pseudostem banana with a p value of 0.001; 0.003; 0,000. In this study there were no significant differences in body weight and jaundice before and after giving nest pseudostem banana with a p value of 0.837; 0.285. Incubators with Nest Pseudostem Banana have a positive impact on premature infants.