Background: Cancer malignancy is often treated by using medication supplements using radio and chemotherapy. However, these treatments have adverse effects on oral which cause difficulties in talking, eating, and even opening the mouth. Therefore, herbal medicine like mangosteen rind gel may become the alternative therapy for this condition.Method: This research was quantitative and experimental randomized pre-post test with control group design. Samples were 15 Sprague Dawley female rats divided into three groups where group 1 was applicated with mangosteen rind gel intervention, unit 2 was with standard gel intervention (positive control) and the last group was untreated group (negative control). Effect of the mangosteen rind gel of treatment group and post-test of the control group were performed with the ANOVA test.Results: The usage of mangosteen rind gel on oral mucositis accelerated the reduction of oral mucositis diameter compared to the standard gel and untreated group. On the 14th day of intervention, the recovery rate of oral mucositis on team 1 reached 99.80%, on unit 2 was 67.70% and only 1.20% in an untreated group. Cell signaling examination showed an average increase of TNF-? from day one until day 14. The lowest difference of TNF-? was group 1 (8.88 pg/mL or 14.50%), then followed by group 2 was 12.71 pg/mL (19.25%), and the highest difference was group 3 (21.43 pg/mL or 32.55%).Conclusion: Mangosteen rind gel may accelerate healing of oral mucositis caused by radio and chemotherapy in cancer treatment. However, further study is necessary to determine the applicability to human being.