Show simple item record

dc.contributorBADAN LITBANGKES KEMENKESid-ID
dc.creatorWigati, R. A.; Puslitbang Ekologi dan Status Kesehatan
dc.creatorMardiana, Mardiana; Puslitbang Ekologi dan Status Kesehatan
dc.creatorMujiyono, Mujiyono; Balai Besar Litbang Vektor dan Reservoir Penyakit Salatiga
dc.creatorAlfiah, Siti; Balai Besar Litbang Vektor dan Reservoir Penyakit Salatiga
dc.date2012-09-23
dc.date.accessioned2019-12-13T03:33:33Z
dc.date.available2019-12-13T03:33:33Z
dc.identifierhttp://ejournal.litbang.depkes.go.id/index.php/MPK/article/view/791
dc.identifier10.22435/mpk.v20i3 Sept.791.
dc.identifier.urihttp://r2kn.litbang.kemkes.go.id:8080/handle/123456789/79911
dc.descriptionAnopheles species as malaria vector, as if tested in the salivary gland containing sporozoites existence which could be checked in the mosquito salivary gland and Enzyme-Linked Immunosorbent Assay (ELISA). This study aimed to investigate the circum sporozoite protein in the mosquito of Anopheles vagus with Enzyme-Linked   Immunosorbent Assay (ELISA). The study was conducted in malaria endemic area namely Hargorejo, Kalirejo, Hargowilis, and Hargotirto villages, Kokap subdistrict, Kulon Progo Regency in June-October 2005. The study design was cross-sectional study. ELISA performed on the An.vagus which is in ovaries shown parous. An.vagus parous body parts for the ELISA are the head-thorax, where it is possible to contain the sporozoites of Plasmodium falciparum or Plasmodium vivax. The results of ELISA to An.vagus in four research areas, showed that 41 samples of An.vagus mosquitoes in Hargorejo village, three positive (7,32%) circum sporozoites protein of Plasmodium falciparum, five samples of An.vagus mosquito in Kalirejo village, there is one positive (20%) circum sporozoites protein of P.falciparum. 16 samples of An.vagus mosquito in Hargowilis village, found one positive (6,25%) circum sporozoite protein of P.falciparum, one sample of An.vagus mosquito in Hargotirto village was not found circum sporozoite protein of P.falciparum (0%) and circum sporozoite protein ofP.vivax (0%). The number of samples from four villages are 63 samples of mosquitoes, found five positive circum sporozoites protein of P.falciparum (7,94%) and not found circum sporozoite protein ofP.vivax (0%). The results of ELISA showed more specimens found positive in mosquitoes containing circum sporozoite protein of P.falciparum in the animal cage, not in human, there were because of choice possibility is not selective. At a wavelength of405 nm in the ELISA results were absorbent for An.vagus mosquitoes tested positive, ranging from 0,257 to 0,632. Value of absorbent positive control was 0,449 and negative control absorbent value were 0,208; 0,228; 0,202; 0,204; 0,207; 0,223; 0,206. The value of cut-off point is taken from the highest absorbent value of negative control is 0,228.   Keywords: Detection,  Circum Sporozoite Protein, An.vagus, Kokap subdistrict, Enzyme-Linked Immunosorbent Assay (ELISA)id-ID
dc.formatapplication/pdf
dc.languageid
dc.publisherBadan Penelitian dan Pengembangan Kesehatanen-US
dc.rightsThe Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Media Penelitian dan Pengembangan Kesehatan (Media of Health Research & Development) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Media Penelitian dan Pengembangan Kesehatan (Media of Health Research & Development) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Media Penelitian dan Pengembangan Kesehatan (Media of Health Research & Development) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.
dc.sourceMedia Penelitian dan Pengembangan Kesehatan; Vol 20, No 3 Sept (2010)en-US
dc.sourceMedia Penelitian dan Pengembangan Kesehatan; Vol 20, No 3 Sept (2010)id-ID
dc.titleDETEKSI PROTEIN CIRCUM SPOROZOITE PADA SPESIES NYAMUK Anopheles vagus TERSANGKA VEKTOR MALARIA DI KECAMATAN KOKAP, KABUPATEN KULON PROGO DENGAN UJI ENZYME-LINKED IMMUNOSORBENTASS A Y (ELISA)id-ID
dc.typePeer-reviewed Articleen-US


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record