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      PELABELAN ANTIBODIANTI-NS1 DENGUE KELINCI DENGAN HORSERADISH PEROKSIDASE

      Date
      2012
      Author
      Paisal, Paisal; UPF Penelitian Kesehatan Aceh
      Dewi, Beti Ernawati; Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Indonesia
      Sudiro, T. Mirawati; Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Indonesia
      Fithriyah, Fithriyah; Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Indonesia
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      Abstract
      Dengue NSI protein can be an ideal target for early detection of dengue virus infection. The aim of the study is to label rabbit anti-NSI antibody with HRP, so that it can be used for detection of NSI protein. The design of this study is laboratory experimental. Anti-NSI IgG-contained rabbit serum was purified with column chromatography (Sephadex G-200). The result of purification was labeled with HRP using periodate method. Then, HRP-labeled IgG was generated with dot blot and ELISA. Using dot blot assay, we found that rabbit anti-NSI IgG labeled with HRP is successful. Nevertheless, the ability of detection was not so good (1:1600). In addition, HRP-labeled IgG used to detect NSI protein utilizing ELISA resulted in high negative control absorbance (0,453 ± 0,013). Therefore, we cannot interpret the assay. The labeling was successful, but it need further optimatization in order to get the HRP-labeled IgG can be used in ELISA. Optimatization was also needed to increasing the ability of detection of HRP-labeled IgG in dot blot assay.   Key words: protein NSI, dengue anti-NSI IgG, labeling, HRP
      URI
      http://ejournal.litbang.depkes.go.id/index.php/MPK/article/view/797
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      Copyright © Badan Litbangkes - Kementerian Kesehatan RI
      Jl. Percetakan Negara no.29, Jakarta Pusat 10560, Indonesia
      All rights reserved. 2019
      Contact Us | Send Feedback