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dc.contributorBADAN LITBANGKES KEMENKESid-ID
dc.creatorPaisal, Paisal; UPF Penelitian Kesehatan Aceh
dc.creatorDewi, Beti Ernawati; Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Indonesia
dc.creatorSudiro, T. Mirawati; Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Indonesia
dc.creatorFithriyah, Fithriyah; Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Indonesia
dc.date2012-09-23
dc.date.accessioned2019-12-13T03:33:39Z
dc.date.available2019-12-13T03:33:39Z
dc.identifierhttp://ejournal.litbang.depkes.go.id/index.php/MPK/article/view/797
dc.identifier10.22435/mpk.v20i4 Des.797.
dc.identifier.urihttp://r2kn.litbang.kemkes.go.id:8080/handle/123456789/79917
dc.descriptionDengue NSI protein can be an ideal target for early detection of dengue virus infection. The aim of the study is to label rabbit anti-NSI antibody with HRP, so that it can be used for detection of NSI protein. The design of this study is laboratory experimental. Anti-NSI IgG-contained rabbit serum was purified with column chromatography (Sephadex G-200). The result of purification was labeled with HRP using periodate method. Then, HRP-labeled IgG was generated with dot blot and ELISA. Using dot blot assay, we found that rabbit anti-NSI IgG labeled with HRP is successful. Nevertheless, the ability of detection was not so good (1:1600). In addition, HRP-labeled IgG used to detect NSI protein utilizing ELISA resulted in high negative control absorbance (0,453 ± 0,013). Therefore, we cannot interpret the assay. The labeling was successful, but it need further optimatization in order to get the HRP-labeled IgG can be used in ELISA. Optimatization was also needed to increasing the ability of detection of HRP-labeled IgG in dot blot assay.   Key words: protein NSI, dengue anti-NSI IgG, labeling, HRPid-ID
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dc.publisherBadan Penelitian dan Pengembangan Kesehatanen-US
dc.rightsThe Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Media Penelitian dan Pengembangan Kesehatan (Media of Health Research & Development) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Media Penelitian dan Pengembangan Kesehatan (Media of Health Research & Development) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Media Penelitian dan Pengembangan Kesehatan (Media of Health Research & Development) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.
dc.sourceMedia Penelitian dan Pengembangan Kesehatan; Vol 20, No 4 Des (2010)en-US
dc.sourceMedia Penelitian dan Pengembangan Kesehatan; Vol 20, No 4 Des (2010)id-ID
dc.titlePELABELAN ANTIBODIANTI-NS1 DENGUE KELINCI DENGAN HORSERADISH PEROKSIDASEid-ID
dc.typePeer-reviewed Articleen-US


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