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dc.contributorBADAN LITBANGKES KEMENKESid-ID
dc.creatorSalwati, Ervi; Puslitbang Pemberantasan Penyakit, Badan Litbangkes
dc.date2012-09-24
dc.date.accessioned2019-12-13T03:34:10Z
dc.date.available2019-12-13T03:34:10Z
dc.identifierhttp://ejournal.litbang.depkes.go.id/index.php/MPK/article/view/832
dc.identifier10.22435/mpk.v12i1 Mar.832.
dc.identifier.urihttp://r2kn.litbang.kemkes.go.id:8080/handle/123456789/79952
dc.descriptionHLA (Human Leukocyte Antigen) contains a set of genes located together on the short arm of chromosome 6. These genes control immune responses, graft acceptance or rejection and tumor surveillance. These abilities have close relationship with genetic variation (occur in "many forms" or alleles) that bind and present antigens to T lymphocytes. Using advanced technology and molecular biology approaches (PCR technique) detection of genetic variation in the HLA region (or HLA typing) has been performed based on DNA.. PCR is an in vitro technique to amplify the DNA sequence enzymatically. "Sequence Specific Primers" (SSP) are designed for this PCR to obtain amplification of specific alleles or groups of alleles. The PCR products are visualized through agarose gel electrophoresis stained with ethidium bromide. The PCR technique requires small amount of whole blood (0.5 - 1 ml), gives rapid, accurate and complete result. This paper discuss identification of HLA class II typing using PCR-SSP technique and show the examples of the results.   Key words: HLA (Human Leukocyte Antigen) class II, PCR (Polymerase Chain Reaction)id-ID
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dc.publisherBadan Penelitian dan Pengembangan Kesehatanen-US
dc.rightsThe Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Media Penelitian dan Pengembangan Kesehatan (Media of Health Research & Development) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Media Penelitian dan Pengembangan Kesehatan (Media of Health Research & Development) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Media Penelitian dan Pengembangan Kesehatan (Media of Health Research & Development) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.
dc.sourceMedia Penelitian dan Pengembangan Kesehatan; Vol 12, No 1 Mar (2002)en-US
dc.sourceMedia Penelitian dan Pengembangan Kesehatan; Vol 12, No 1 Mar (2002)id-ID
dc.titleIDENTIFIKASI TIPE HLA KELAS II DENGAN TEKNIK PCRid-ID
dc.typePeer-reviewed Articleen-US


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