One of the gastrointestinal nosocomial infections that usually occurs at pediatric wards is diarrhoea/gastroenteritis. The objective of this study was to know the occurrence of gastrointestinal nosocomial infection (GNI) cases in 3 hospitals in Jakarta. As unit samples were all infants and children with gastroenteritis in one month period and 31 nurses. Rectal swabs were taken twice, when they were entering the hospital and 3 days after nursing, while hand-swabs were taken from 31 nurses in the pediatric ward. If the faeces of infants and children contained pathogenic bacteria (E. coli, Salmonella sp., Pseudomonas sp., Staphylococcus sp.) on the third day which was not found an first day, it means that they suffered from GNI. The microbiological test showed that of those 96 patients, 13.54% were suffering from GNI and the cause of GNI was E. coli (76.9%). While hand-swabs of nurses test showed that 3.2% contained E. coli, 6.4% Pseudomonas sp, and 12.9% Staphylococcus sp.