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dc.contributorBADAN LITBANGKES KEMENKESid-ID
dc.creatorGlover, Janet; Department of Biological Sciences, Smith College, Northampton Massachusetts, USA
dc.creatorWilliams, Steven A.; Department of Biological Sciences, Smith College, Northampton Massachusetts, USA
dc.creatorSzabo, Susanne; Department of Biological Sciences, Smith College, Northampton Massachusetts, USA
dc.creatorLandry, David; New England Biolabs, 32 Tozer Road, Beverly, Massachusetts, USA
dc.creatorMcReynolds, Larry A.; New England Biolabs, 32 Tozer Road, Beverly, Massachusetts, USA
dc.creatorSupali, Taniawati; Department of Parasitology, University of Indonesia, Jakarta
dc.creatorPartono, Felix; Department of Parasitology, University of Indonesia, Jakarta
dc.date2012-09-20
dc.date.accessioned2019-12-16T09:31:00Z
dc.date.available2019-12-16T09:31:00Z
dc.identifierhttp://ejournal.litbang.depkes.go.id/index.php/BPK/article/view/643
dc.identifier10.22435/bpk.v17i2 Jun.643.
dc.identifier.urihttp://r2kn.litbang.kemkes.go.id:8080/handle/123456789/80541
dc.descriptionBlood samples from 43 humans and 14 cats positive with Brugia microfilariae were analyzed in a field study in Tanjung Pinang, Indonesia. The study used the polymerase chain reaction (PCR) to compare the sensitivity of radioactive and biotinylated species-specific oligonuleotide probes. The cloning char­acterization of the Hha I repeat DNA family found in filarial parasites of the genus Brugia, and the development of species-specific probes for B.malayi and B.pahangi based on these repeats has been described elsewhere (PNAS USA 83: 797-801); Mol.Biochem. Parasitol. 2$: 163-170). The use of radioisotopes for labelling DNA probes is both expensive and inconvenient. To replace these probes, biotinylated DNA probes have been designed for non- radioactive detection of B.malayi and B.pahangi. These oligonucleotide probes have long tails of biotinylated uridine residues added to their 5' end. As little as 100 pg of Brugia DNA can be detected on dot blot with these probes. Detection of the probes is based on an avidin-alkaline phosphatase colorimetric assay. In order to distinguish between infected from uninfected individuals, it is necessary to detect the amount of DNA in one microfilaria (about 60 pg). The polymerase chain reaction (PCR) is a procedure in which a small amount of DNA can be amplified up to 1 million-fold. A part of each sample in this study was PCR amplified and compared with the unamplified portion using both the radioactive and biotinylated DNA probe. The PCR amplified samples were accurately identified by both the radioactive and biotinylated B.malayi and B.pahangi probes. Even samples with as few as two microfilariae per lOOul of blood were easily detected. The samples that were not PCR amplified were accurately identified after only long exposures (greater than one week) to the radioactive probes. The biotinylated probes, were not sensitive enough for accurate identification of the non-PCR amplified samples. The polymerase chain reaction is, therefore, a promising new tool for enhancing the sensitivity of parasite detection assays based on DNA probes. This will be especially important in designing assay based on non-radioactive DNA probes.id-ID
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dc.languageid
dc.publisherBadan Penelitian dan Pengembangan Kesehatanen-US
dc.rightsThe Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.
dc.sourceBuletin Penelitian Kesehatan; Vol 17, No 2 Jun (1989)en-US
dc.subjectHealth; Kesehatanid-ID
dc.subjectPolymerase Chain Reaction (PCR); Brugia Microfilariaeid-ID
dc.subjectid-ID
dc.titleA FIELD STUDY USING THE POLYMERASE CHAIN REACTION (PCR) TO SCREEN FOR BRUGIA MICROFILARIAE IN HUMAN AND ANIMAL BLOODid-ID
dc.typeid-ID
dc.typeen-US


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