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dc.contributorid-ID
dc.creatorRinendyaputri, Ratih; Pusat Biomedis dan Teknologi Dasar Kesehatan Balitbangkes, Kemenkes RI
dc.creatorBoediono, Arief; Departemen Anatomi, Fakultas Kedokteran Hewan Institut Pertanian Bogor
dc.date2013-11-08
dc.date.accessioned2019-12-16T09:33:15Z
dc.date.available2019-12-16T09:33:15Z
dc.identifierhttp://ejournal.litbang.depkes.go.id/index.php/BPK/article/view/3288
dc.identifier10.22435/bpk.v41i3 Sep.3288.171-178
dc.identifier.urihttp://r2kn.litbang.kemkes.go.id:8080/handle/123456789/80779
dc.descriptionAbstract Application or the basic research of the stem cells is continously conducted to explore their potential, particularly the use of stem cells in health. Cryopreservation technology is needed to maintain availability of stem cell. This study was  aimed to observe the development of murine embryonic stem cell (ESC) primary colonies post-vitrified inner cell mass (ICM). Inner cell mass (ICM) was derived from blastocysts of murine embryos of female Swiss webster mice using pregnant mare’s serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG). Inner Cell Mass (ICM) was isolated  from blastocysts by immunosurgery. Inner Cell Mass (ICM) were divided into two groups: the control and vitrified ICM. Both groups were cultured and observed on the ICM attachment (attachment rate/AR), the primary formation of ESC colonies (primary colony/PC) and  diameter of primary colony ESC which grouped into small, medium and large. The results showed that AR and medium diameter’s group of primary colony ESC were lower (p<0.05) in vitrified ICM than control ICM. However the percentage of PC and diameter of primary colonies ESC in small and large groups were not different compared with control (p>0.05). This study shows that vitrification for cryopreserving ICM can be used as an alternative source of ESC. Key words: blastocyst, inner cell mass, ICM, vitrification Abstrak  Submit : 30-01-2013  Review : 08-02-2013 Review : 11-03-2013 revisi : 26–03-2013 Aplikasi maupun penelitian dasar untuk menggali potensi sel punca khususnya pemanfaatan sel punca di bidang kesehatan terus dilakukan. Teknologi kriopreservasi dibutuhkan untuk menjaga ketersediaan sel punca. Penelitian ini bertujuan mengamati perkembangan koloni primer embryonic stem cell (ESC) mencit pasca vitrifikasi inner cell mass (ICM). Inner Cell Mass (ICM) diperoleh dari embrio blastosis mencit betina Swis webster menggunakan pregnant mare’s serum gonadotropin (PMSG) dan human chorionic gonadotropin (hCG). ICM diisolasi dari embrio blastosis menggunakan metode immunosurgery. ICM yang diperoleh dibagi menjadi dua kelompok yaitu ICM kontrol dan ICM vitrifikasi. Kedua kelompok dikultur serta dilakukan pengamatan terhadap tingkat perlekatan (attachment rate/AR) ICM, tingkat pembentukan koloni primer ESC (primary colony/PC) dan diameter koloni primer ESC yang dikategorikan menjadi 3 kelompok yaitu kecil, sedang dan besar. Hasil menunjukkan bahwa ICM vitrifikasi mempunyai persentase AR dan diameter koloni primer ESC pada kelompok sedang lebih rendah (p<0,05) terhadap ICM kontrol. Namun persentase PC serta diameter koloni primer ESC kelompok kecil dan besar pada ICM vitrifikasi tidak menunjukkan perbedaan (P>0,05) terhadap kontrol. Penelitian ini menunjukkan bahwa vitrifikasi dapat digunakan untuk kriopreservasi ICM sebagai sumber ESC.   Kata kunci : blastosis, inner cell mass, ESC, vitrifikasiid-ID
dc.formatapplication/pdf
dc.languageid
dc.publisherBadan Penelitian dan Pengembangan Kesehatanen-US
dc.rightsThe Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.
dc.sourceBuletin Penelitian Kesehatan; Vol 41, No 3 Sep (2013); 171-178en-US
dc.subjectblastocyst, inner cell mass, ICM, vitrificationid-ID
dc.subjectblastocyst, inner cell mass, ICM, vitrificationid-ID
dc.subjectblastocyst, inner cell mass, ICM, vitrificationid-ID
dc.titlePERKEMBANGAN KOLONI PRIMER EMBRYONIC STEM CELL (ESC) MENCIT PASCA VITRIFIKASI INNER CELL MASS (ICM)id-ID
dc.typeid-ID
dc.typeen-US


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