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dc.contributorid-ID
dc.creatorSoekarso, Triyani; Center for Biomedical and Basic Technology of Health, National Institute of Health Research and Development, Ministry of Health of Indonesia
dc.creatorSetiawaty, Vivi; Center for Biomedical and Basic Technology of Health, National Institute of Health Research and Development, Ministry of Health of Indonesia
dc.date2013-05-23
dc.date.accessioned2019-12-17T00:36:19Z
dc.date.available2019-12-17T00:36:19Z
dc.identifierhttp://ejournal.litbang.depkes.go.id/index.php/HSJI/article/view/3073
dc.identifier10.22435/hsji.v3i2 Des.3073.91-94
dc.identifier.urihttp://r2kn.litbang.kemkes.go.id:8080/handle/123456789/80975
dc.descriptionAbstrakLatar belakang: Rotavirus adalah agen etiologi yang paling umum sebagai penyebab diare berat pada bayi dan anak-anak di seluruh dunia. Secara global, setiap tahun terjadi 600.000 kematian pada anak-anak kurang dari 5 tahun terkait dengan infeksi rotavirus. Spesimen yang digunakan untuk mendeteksi Rotavirus biasanya feses, tetapi untuk mendapatkan spesimen feses lebih sulit dibandingkan usap dubur. Untuk itu perlu adanya perbandingan hasil identifikasi rotavirus dari usap dubur dengan feses yang diuji dengan metode RT-PCR.Metode: Melakukan identifikasi rotavirus yang menyebabkan diare dengan menggunakan spesimen usap dubur dan feses yang diambil dari anak balita dan diperiksa dengan metode RT-PCR. Data dianalisis dengan uji sensitifitas dan spesifisitas.Hasil:Hasil RT-PCR rotavirus dari 189 pasangan spesimen didapat 24 adalah negatif pada kedua jenis spesimen dan 112 adalah positif pada kedua jenis spesimen, 42 pasangan positif pada spesimen tinja saja dan 11 pasangan positif pada usap dubur saja. Hasil sensitivitas sampel usap dubur adalah 72,7% dan spesifisitasnya 68,6 %. Nilai penduga positif usap dubur sebesar 91,1% sedangkan nilai penduga negatif sebesar 36,3%.Kesimpulan:Hasil pemeriksaan sensitifitas dan spesifisitas  identifikasi rotavirus dari usap dubur masih memadai dibandingkan dengan specimen feses. (Health Science Indones 2012;2:xx-xx)Kata kunci:RT-PCR, rotavirus, rectal swab, feces, diarrhea symptomsAbstractBackground: Rotavirus is the most common etiologic agent causes severe diarrhea in infants and children worldwide. Globally, every year 600,000 deaths in children less than 5 years associated with rotavirus infection. Commonly used to detect rotavirus stool samples, but getting stool samples were more difficult than rectal swab. The purpose of this study was to compare the results of the use of specimen of rectal swabs and feces to detect Rotavirus by RT-PCR method.Methods: To evaluate rotavirus that cause diarrhea we used rectal swabs and stool samples taken from infants and identified by RT-PCR method. Data were analyzed with sensitivity and specificity analysis tests.Results:A number 189 specimen pairs were included of which 24 were negative in both specimen types and 112 were positive in both specimen types. Forty four 42 pairs was positive in the stool specimen only and 11 pairs was positive in the rectal swab specimen only. Sensitivity of rectal swab specimen was 72.7% and specificity was 68.6%. Rectal swab positive predictive value of 91.1%, while a negative predictive value of 36.3%.Conclusion:The result of the sensitivity and specificity of rectal swab specimen was adequate compared with the feces specimen. (Health Science Indones 2012;2:xx-xx)Key words:RT-PCR, rotavirus, rectal swabs, stool, diarrhea symptomsid-ID
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dc.languageid
dc.publisherBadan Penelitian dan Pengembangan Kesehatanen-US
dc.rightsThe Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.
dc.rightsUnder the terms of Indonesian Goverment Copyright Law, Health Science Journal of Indonesia must formally obtain transfer of copyright before an article can be published. Health Science Journal of Indonesia grants to the author (and to all coauthors) the right to present orally in any forum all or part of the work. It is also stipulated that all graphics including figures, tables and charts may be republished with permission from Health Science Journal of Indonesia. In consideration of the fact that Health Science Journal of Indonesia undertakes to publish my article: I affirm that the material has not been previously published and that I (and my coauthors) own and have not transferred elsewhere any rights to the article.I affirm that I (and my coauthors) have obtained written permission to use any previously copyrighted material included in the article and that such documentation will be forwarded to Health Science Journal of Indonesia simultaneously with the article. I further affirm that I (and my coauthors) have stated any possible conflicts of interest within the article.I (and my coauthors) hereby assign and transfer to Health Science Journal of Indonesia all rights of copyright ownership and permission to the article, including without limitation or restriction, all rights of reproduction, derivation, distribution, sale, reuse, and display of the of the work, in whole or in part, including recompilation and stand-alone publication, in any and all forms of media now or hereafter known, including all electronic and digital media, as protected by the laws of the Republic of Indonesia and foreign countries and to authorize others to make such uses of the work. These rights will become the property of Health Science Journal of Indonesia from the date of acceptance of the article for publication and extend for the life of the copyright. I understand that Health Science Journal of Indonesia, as copyright owner, has authority to grant permission to reproduce the article.
dc.sourceHealth Science Journal of Indonesia; Vol 3, No 2 Des (2012); 91-94en-US
dc.sourceHealth Science Journal of Indonesia; Vol 3, No 2 Des (2012); 91-94id-ID
dc.subjectid-ID
dc.subjectRT-PCR, rotavirus, rectal swabs, stool, diarrhea symptomsid-ID
dc.subjectid-ID
dc.titleComparison of rotavirus detection from rectal swab and feces in patients with diarrhea symptomsid-ID
dc.typePeer-reviewed Articleen-US


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