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dc.contributorid-ID
dc.creatorSoleha, Maratu; Center for Biomedical and Applied Health Technology, National Institute of Health Research and Development, Ministry of Health Republic Indonesia
dc.date2013-07-04
dc.date.accessioned2019-12-17T00:36:24Z
dc.date.available2019-12-17T00:36:24Z
dc.identifierhttp://ejournal.litbang.depkes.go.id/index.php/HSJI/article/view/3096
dc.identifier10.22435/hsji.v4i1 Jun.3096.41-46
dc.identifier.urihttp://r2kn.litbang.kemkes.go.id:8080/handle/123456789/80986
dc.descriptionAbstrakLatar belakang: Bakteri Escherichia coli (E. coli) ada yang telah bermutasi menjadi patogen yang menimbulkan berbagai penyakit seperti hemorrhagic colitis (HC), hemolytic uremic syndrome (HUS), sepsis, pnemonia, neonatal meningitis, dan infeksi saluran kemih. Mutasi terjadi karena bakteri ini menerima transfer gen yang virulen dari bakteri lain yang hidup di sekitarnya. E. coli yang biasanya hidup normal di dalam usus manusia telah beradaptasi sehingga bisa hidup di tanah, makanan, dan saluran kemih. Penelitian ini mendeteksi gene yang virulen pada DNA isolat E. coli. Metode: Untuk deteksi E. coli yang virulen pada penelitian ini digunakan metode Real-time PCR dengan mencocokkan hasil sekuensing dengan sekuens E. coli virulen yang telah di publikasikan sebagai rujukan. Hasil: Sekuens RT PCR menggambarkan DNA gen eae pada BLAST mempunyai kesesuaian dengan rujukan segmen E. coli yang virulen. Dari sampel yang berasal dari E. coli di sekitar perairan lingkungan didapatkan gen Eae sebagai gen yang menyebabkan E. coli menjadi virulen sebesar 7,3%. Kesimpulan: E. coli yang virulen ditemukan pada sampel E. coli yang berasal dari perairan lingkungan dengan metode realtime PCR. (Health Science Indones 2013;1:41-6) Kata kunci: gen virulen E. coli, real-time PCR, perairan lingkunganAbstractBackground: Escherichia coli(E. coli) bacteria have developed into pathogenic bacteria that caused diseases such as hemorrhagic colitis (HC), hemolytic uremic syndrome (HUS), sepsis, pneumonia, neonatal meningitis, and urinary tract infections. Pathogenic E. coli have acquired pathogenic/virulence genes from other bacteria in their environment. E. coli that normally lived in the human gut had adapted to other niches such as soil, food and the urinary tract. This study investigated the presence of pathogenic E. coli from water samples by examining E. coli virulence genes present in E. coli genomes of water sourced isolates. Methods:This study used Real-time PCR to detect virulent E. coli and sequencing which was then matched to virulent E. coli listed in BLAST as a reference.Results: E. coli sequence from RT PCR was matched with eae DNA segment pattern in BLAST. E. coli isolates tested with eae gene were detected in 7.3% of E. coli strains isolated from environmental water sources. Conclusion:The presence of virulent E. coli strains was detected in environmental water sources using Real time PCR. (Health Science Indones 2013;1:41-6) Keywords: E. coli virulence genes, real-time PCR, environmental waterid-ID
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dc.publisherBadan Penelitian dan Pengembangan Kesehatanen-US
dc.rightsThe Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.
dc.rightsUnder the terms of Indonesian Goverment Copyright Law, Health Science Journal of Indonesia must formally obtain transfer of copyright before an article can be published. Health Science Journal of Indonesia grants to the author (and to all coauthors) the right to present orally in any forum all or part of the work. It is also stipulated that all graphics including figures, tables and charts may be republished with permission from Health Science Journal of Indonesia. In consideration of the fact that Health Science Journal of Indonesia undertakes to publish my article: I affirm that the material has not been previously published and that I (and my coauthors) own and have not transferred elsewhere any rights to the article.I affirm that I (and my coauthors) have obtained written permission to use any previously copyrighted material included in the article and that such documentation will be forwarded to Health Science Journal of Indonesia simultaneously with the article. I further affirm that I (and my coauthors) have stated any possible conflicts of interest within the article.I (and my coauthors) hereby assign and transfer to Health Science Journal of Indonesia all rights of copyright ownership and permission to the article, including without limitation or restriction, all rights of reproduction, derivation, distribution, sale, reuse, and display of the of the work, in whole or in part, including recompilation and stand-alone publication, in any and all forms of media now or hereafter known, including all electronic and digital media, as protected by the laws of the Republic of Indonesia and foreign countries and to authorize others to make such uses of the work. These rights will become the property of Health Science Journal of Indonesia from the date of acceptance of the article for publication and extend for the life of the copyright. I understand that Health Science Journal of Indonesia, as copyright owner, has authority to grant permission to reproduce the article.
dc.sourceHealth Science Journal of Indonesia; Vol 4, No 1 Jun (2013); 41-46en-US
dc.sourceHealth Science Journal of Indonesia; Vol 4, No 1 Jun (2013); 41-46id-ID
dc.subjecthealthid-ID
dc.subjectE. coli virulence genes, real-time PCR, environmental waterid-ID
dc.subjectHealthid-ID
dc.titleDetection of attaching and effacing virulence gene of E. coliid-ID
dc.typePeer-reviewed Articleen-US


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