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dc.contributoren-US
dc.creatorAdam, Kindi; Center for Biomedical and Basic Technology of Health, National Institute for Health Research and Development, Ministry of Health of Indonesia
dc.creatorSetiawaty, Vivi; Center for Biomedical and Basic Technology of Health, National Institute for Health Research and Development, Ministry of Health of Indonesia
dc.date2015-01-06
dc.date.accessioned2019-12-17T00:36:36Z
dc.date.available2019-12-17T00:36:36Z
dc.identifierhttp://ejournal.litbang.depkes.go.id/index.php/HSJI/article/view/3595
dc.identifier10.22435/hsji.v5i2 Dec.3595.83-88
dc.identifier.urihttp://r2kn.litbang.kemkes.go.id:8080/handle/123456789/81003
dc.descriptionAbstrakLatar belakang: Virus avian influenza (AI) H5N1 telah menyebabkan kematian hampir 600 orang di dunia sejak tahun 2003. Virus AI H5N1 masih beredar pada unggas liar dan domestik. Salah satu sifat patogenitas virus AI  diketahui dari kemampuan protein nonstructural (NS1 dan NS2) dalam menginduksi badai sitokin. Mutasi yang terjadi pada protein NS1 dan NS2 dapat memperberat infeksi yang terjadi. Metode: Sekuens genetik gen NS yang mengkode domain C-terminus dari virus AI H5N1 didapat dengan metode sekuensing menggunakan primer khusus untuk mengamplifikasi gen NS. Desain primer dilakukan dengan menggunakan beberapa software bioinformatika yang tersedia kemudian primer diuji kembali untuk mengetahui keberhasilan dalam mengamplifikasi sekuen target. Pengujian RT-PCR dilakukan pada empat isolat virus AI H5N1, dilanjutkan dengan sekuensing untuk mengetahui urutan basa nukleotida yang dihasilkan dari primer yang dibuat dalam penelitian ini. Hasil: Primer yang didesain dapat mengamplifikasi empat isolat H5N1 ayam yang diuji yaitu dua isolat dari Bali (A/Ck/Bali-KH/2009 dan A/Ck/Bali-T/2009),serta dua isolat dari Jawa Timur (A/Ck/Jawa Timur-S/2009 dan A/Ck/Jawa Timur-D/2009).Kesimpulan: Primer yang didesain dapat digunakan dalam amplifikasi PCR dan sekuensing gen NS yang mengekspresikan bagian C terminus dari protein NS1 dan bagian ujung NS2. (Health Science Indones2014;2:83-8)Kata kunci: avian influenza, H5N1, gen NS, C-terminusAbstractBackground: The avian influenza (AI) H5N1 viruses have caused nearly 600 fatal cases in the world since 2003. The H5N1 AI virus is still circulating in wild birds and domestic. One of the known of AI virus pathogenicity is the ability of the nonstructural protein (NS1 and NS2) to induce cytokine storm. Mutations of the NS1 and NS2 proteins may aggravate infections.Methods: Genetic sequences of NS gene that encodes the C - terminus domain of AI H5N1 virus were obtained by sequencing method using specific primer to amplify the NS gene. The primers design were done by using several bioinformatics software available then retested to determine the primer success in amplifying the target sequence. RT- PCR testing performed on four H5N1 AI virus isolates, followed by sequencing to determine the nucleotide sequences generated from the primer were prepared in this study. Results: Primers, which can be designed to amplify four chicken H5N1 isolates tested were two isolates from Bali (A/Ck/Bali-KH/2009 and A/Ck/Bali-T/2009), and two isolates from East Java (A/Ck/East Java-S/2009 and A/Ck/East Java-D/2009). Conclusion: The designated primer can be used in PCR amplification and sequencing of genes that express NS section C terminus of the protein NS1 and NS2 end portion. The primer design of H5N1 NS gene. (Health Science Indones 2014;2:83-8)Keywords: avian influenza, H5N1, NS gene, C-terminusen-US
dc.formatapplication/pdf
dc.languageen
dc.publisherBadan Penelitian dan Pengembangan Kesehatanen-US
dc.relationhttp://ejournal.litbang.depkes.go.id/index.php/HSJI/article/downloadSuppFile/3595/9475
dc.relationhttp://ejournal.litbang.depkes.go.id/index.php/HSJI/article/downloadSuppFile/3595/9476
dc.rightsThe Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.
dc.rightsUnder the terms of Indonesian Goverment Copyright Law, Health Science Journal of Indonesia must formally obtain transfer of copyright before an article can be published. Health Science Journal of Indonesia grants to the author (and to all coauthors) the right to present orally in any forum all or part of the work. It is also stipulated that all graphics including figures, tables and charts may be republished with permission from Health Science Journal of Indonesia. In consideration of the fact that Health Science Journal of Indonesia undertakes to publish my article: I affirm that the material has not been previously published and that I (and my coauthors) own and have not transferred elsewhere any rights to the article.I affirm that I (and my coauthors) have obtained written permission to use any previously copyrighted material included in the article and that such documentation will be forwarded to Health Science Journal of Indonesia simultaneously with the article. I further affirm that I (and my coauthors) have stated any possible conflicts of interest within the article.I (and my coauthors) hereby assign and transfer to Health Science Journal of Indonesia all rights of copyright ownership and permission to the article, including without limitation or restriction, all rights of reproduction, derivation, distribution, sale, reuse, and display of the of the work, in whole or in part, including recompilation and stand-alone publication, in any and all forms of media now or hereafter known, including all electronic and digital media, as protected by the laws of the Republic of Indonesia and foreign countries and to authorize others to make such uses of the work. These rights will become the property of Health Science Journal of Indonesia from the date of acceptance of the article for publication and extend for the life of the copyright. I understand that Health Science Journal of Indonesia, as copyright owner, has authority to grant permission to reproduce the article.
dc.sourceHealth Science Journal of Indonesia; Vol 5, No 2 Dec (2014); 83-88en-US
dc.sourceHealth Science Journal of Indonesia; Vol 5, No 2 Dec (2014); 83-88id-ID
dc.subjecten-US
dc.subjectavian influenza, H5N1, NS gene, C-terminusen-US
dc.subjecten-US
dc.titleThe primer design of domain-specific C-terminus of the avian H5N1 NS gene from Indonesiaen-US
dc.typePeer-reviewed Articleen-US


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