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dc.creatorYunarto, Nanang
dc.creatorAini, Nurul
dc.descriptionAbstrak Latar belakang: Aterosklerosis merupakan faktor risiko timbulnya penyakit jantung koroner (PJK). Senyawakatekin memiliki aktivitas antioksidan tinggi yang dapat menghambat terjadinya aterosklerosis. Ekstrak daungambir (Uncaria gambir, Roxb.) mengandung katekin tinggi sehingga berpotensi menghambat terjadinyaaterosklerosis. Penelitian ini bertujuan untuk menguji efek ekstrak terpurifikasi daun gambir dalam menghambataterosklerosis pada tikus.Metode: Desain penelitian ini adalah eksperimental laboratorium yang dilakukan di Laboratorium Farmasidan Laboratorium Hewan, Badan Litbangkes, Kemenkes RI pada tahun 2014. Ekstrak gambir dipurifikasi untukdiperoleh kandungan katekin yang optimal, selanjutnya dilakukan uji aktivitas antioksidan menggunakan pereaksi2,2-difenil-1-pikrilhidrazil (DPPH) dengan pembanding asam askorbat. Penelitian ini menggunakan 36 ekortikus putih jantan galur Sprague Dawley berusia 2,5 bulan yang dibagi secara acak ke dalam enam kelompokyaitu kelompok normal, kontrol negatif (akuades), kontrol positif (atorvastatin 2 mg/200 g bb), ekstrak dosis I (20mg/200 g bb), dosis II (40 mg/200 g bb) dan dosis III (80 mg/200 g bb). Tikus diinduksi dengan makanan yangmengandung lemak tinggi dan perlakuan pengobatan sesuai kelompoknya selama 60 hari, kecuali kontrol normal.Hasil: Kadar katekin dalam ekstrak gambir terpurifikasi diperoleh sebesar 92,69%. Dari uji aktivitasantioksidan diperoleh IC50 11,76 μg/mL. Hasil pengukuran aktivitas antiateroskelrosis menunjukkan bahwa jikadibandingkan dengan kontrol negatif, ketiga dosis ekstrak gambir terpurifikasi mampu mencegah terjadinyaaterosklerosis dengan menghambat penebalan dinding aorta dan pembentukan sel busa (p<0,05). Aktivitasantiaterosklerosis meningkat dengan bertambahnya dosis.Kesimpulan: Ekstrak gambir terpurifikasi mempunyai efek mencegah penebalan dinding dan pembentukansel busa aorta tikus. (Health Science Journal of Indonesia 2015;6:105-10)Kata kunci: gambir, katekin, antiaterosklerosis AbstractBackground: Atherosclerosis is a risk factor for coronary heart disease (CHD). Catechin have highantioxidant activity that can prevent atherosclerosis. Gambir (Uncaria gambir, Roxb.) leaves extract havehigh catechin content thereby potentially inhibiting atherosclerosis. This research was aimed to examineeffect of purified gambir leaves extract to prevent atherosclerosis in rats.Methods: The experimental laboratory study was conducted in Pharmacy Laboratory and Animal Laboratory,National Institute of Health Research and Development, Ministry of Health, Republic of Indonesia in 2014.Gambir leaves extract were purified to gain optimum catechin. Afterwards, antioxidant activity was testedusing 2.2-diphenyl-1-picrylhydrazyl (DPPH) method, with ascorbic acid as positive control. Thirty six whitemale Sprague Dawley rats aged 2.5 months were randomly divided into six groups, i.e. normal control group,negative control group (aquadest), positive control group (atorvastatin 2 mg/200 g bw),extract dose I (20mg/200 g bw), dose II (40 mg/200 g bw) and dose III (80 mg/200 g bw). The rats were given high fat diet andtreatment according to their group for 60 days, except for normal control group.Results: Catechin content in the purified gambir leaves extract was 92,69%. From antioxidant activity test, IC50 wasfound to be 11,76 μg/mL. Anti-atherosclerotic activity study shown that compared to negative control, all three dosesof purified gambir leaves extract were able to prevent atherosclerosis through inhibition of aortic wall thickening andfoam cell formation due to high fat diet (p<0.05). Anti-atherosclerotic activity increased with increasing dose.Conclusion: Gambir leaves purified extract had the effect of preventing the thickening of the walls andfoam cell formation rat aorta. (Health Science Journal of Indonesia 2015;6:105-10)Keywords: gambir, catechin, antiatherosclerosisen-US
dc.publisherBadan Penelitian dan Pengembangan Kesehatanen-US
dc.rightsThe Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.
dc.rightsUnder the terms of Indonesian Goverment Copyright Law, Health Science Journal of Indonesia must formally obtain transfer of copyright before an article can be published. Health Science Journal of Indonesia grants to the author (and to all coauthors) the right to present orally in any forum all or part of the work. It is also stipulated that all graphics including figures, tables and charts may be republished with permission from Health Science Journal of Indonesia. In consideration of the fact that Health Science Journal of Indonesia undertakes to publish my article: I affirm that the material has not been previously published and that I (and my coauthors) own and have not transferred elsewhere any rights to the article.I affirm that I (and my coauthors) have obtained written permission to use any previously copyrighted material included in the article and that such documentation will be forwarded to Health Science Journal of Indonesia simultaneously with the article. I further affirm that I (and my coauthors) have stated any possible conflicts of interest within the article.I (and my coauthors) hereby assign and transfer to Health Science Journal of Indonesia all rights of copyright ownership and permission to the article, including without limitation or restriction, all rights of reproduction, derivation, distribution, sale, reuse, and display of the of the work, in whole or in part, including recompilation and stand-alone publication, in any and all forms of media now or hereafter known, including all electronic and digital media, as protected by the laws of the Republic of Indonesia and foreign countries and to authorize others to make such uses of the work. These rights will become the property of Health Science Journal of Indonesia from the date of acceptance of the article for publication and extend for the life of the copyright. I understand that Health Science Journal of Indonesia, as copyright owner, has authority to grant permission to reproduce the article.
dc.sourceHealth Science Journal of Indonesia; Vol 6, No 2 (2015); 105-110en-US
dc.sourceHealth Science Journal of Indonesia; Vol 6, No 2 (2015); 105-110id-ID
dc.titleEffect of purified gambir leaves extract to prevent atherosclerosis in ratsen-US
dc.typePeer-reviewed Articleen-US

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