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dc.contributoren-US
dc.creatorSubangkit, Subangkit; Research and Development Center for Biomedical and Basic Health Technology, National Insitute of Health Research and Development, Jakarta, Indonesia
dc.creatorMursinah, Mursinah; Research and Development Center for Biomedical and Basic Health Technology, National Insitute of Health Research and Development, Jakarta, Indonesia
dc.creatorPutranto, Rudi Hendro; Research and Development Center for Health Resources and Services, National Insitute of Health Research and Development, Jakarta, Indonesia
dc.creatorSetiawaty, Vivi; Research and Development Center for Biomedical and Basic Health Technology, National Insitute of Health Research and Development, Jakarta, Indonesia
dc.date2017-07-17
dc.date.accessioned2019-12-17T00:37:16Z
dc.date.available2019-12-17T00:37:16Z
dc.identifierhttp://ejournal.litbang.depkes.go.id/index.php/HSJI/article/view/6445
dc.identifier10.22435/hsji.v8i1.6445.7-11
dc.identifier.urihttp://r2kn.litbang.kemkes.go.id:8080/handle/123456789/81041
dc.descriptionLatar Belakang: Campak adalah salah satu penyakit menular dan dapat menyebabkan penyakit serius sampai kematian. Campak masih merupakan masalah kesehatan masyarakat karena wabah campak masih terjadi di seluruh Indonesia. Surveilans berbasis laboratorium berikut penyelidikan epidemiologi molekuler memiliki kontribusi besar untuk mencegah wabah campak. Studi sebelumnya telah mendokumentasikan kehadiran genotipe virus campak G2, G3 dan D9 di Indonesia, dan genotipe lainnya seperti B3, D4, D5, D8 dan H1 telah terdeteksi di negara-negara tetangga. Penelitian ini bertujuan untuk mengkarakterisasi virus campak yang menyebabkan wabah di Indonesia pada tahun 2014. Metode: Tujuh puluh empat spesimen urin yang dikumpulkan dari delapan provinsi dan diperiksa oleh satu langkah RT-PCR dan metode sequencing Sanger. Analisis sekuensing dilakukan menggunakan Bioedit 7.1; DNAstar 7.0 dan software MEGA5.0. Hasil: Hasil PCR menunjukkan 34 dari 74 spesimen klinis positif dari virus campak. Kami menemukan genotipe dari 34 virus campak milik genotipe D8, D9 dan G3. Kesimpulan: Campak pertama genotipe D8 telah terdeteksi dari Indonesia pada tahun 2014 meskipun campak lainnya genotipe masih dapat ditemukan di Indonesia. Kata kunci: virus campak, D8 genotipe, wabah, Indonesia Background: Measles is a highly contagious viral disease. It remains an important cause of death among young children globally, despite the availability of a safe and effective vaccine. Measles is still a public health problem in Indonesia and measles outbreak still reported from many areas  throughout Indonesia.. Molecular epidemiology of measles viruses is an important component in outbreak investigations to to monitor the presence of circulating wild–type measles strains.Previous studies in Indonesia have documented the presence of measles virus genotypes G2, G3 and D9 in Indonesia, and the other genotypes such as B3, D4, D5, D8 and H1 have been detected in neighboring countries. This study aims to characterize the measles virus that causing outbreak in Indonesia in 2014. Methods: Seventy four urine specimens were collected from eight provinces and examined by one step RT-PCR and Sanger sequencing method. Sequencing analysis were conducted using Bioedit 7.1; DNA Star 7.0 and MEGA 5.0 software. Results: The PCR results showed 34 out of 74 clinical specimens positive of measles virus. We found the genotype of 34 measles viruses belongs to genotype D8, D9 and G3. Conclusion: The first measles genotype D8 has been detected from Indonesia in 2014 although other measles genotype still can be found in Indonesia. Keywords: Measles virus, D8 genotype, outbreak, Indonesia  en-US
dc.formatapplication/pdf
dc.languageen
dc.publisherBadan Penelitian dan Pengembangan Kesehatanen-US
dc.rightsThe Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.
dc.rightsUnder the terms of Indonesian Goverment Copyright Law, Health Science Journal of Indonesia must formally obtain transfer of copyright before an article can be published. Health Science Journal of Indonesia grants to the author (and to all coauthors) the right to present orally in any forum all or part of the work. It is also stipulated that all graphics including figures, tables and charts may be republished with permission from Health Science Journal of Indonesia. In consideration of the fact that Health Science Journal of Indonesia undertakes to publish my article: I affirm that the material has not been previously published and that I (and my coauthors) own and have not transferred elsewhere any rights to the article.I affirm that I (and my coauthors) have obtained written permission to use any previously copyrighted material included in the article and that such documentation will be forwarded to Health Science Journal of Indonesia simultaneously with the article. I further affirm that I (and my coauthors) have stated any possible conflicts of interest within the article.I (and my coauthors) hereby assign and transfer to Health Science Journal of Indonesia all rights of copyright ownership and permission to the article, including without limitation or restriction, all rights of reproduction, derivation, distribution, sale, reuse, and display of the of the work, in whole or in part, including recompilation and stand-alone publication, in any and all forms of media now or hereafter known, including all electronic and digital media, as protected by the laws of the Republic of Indonesia and foreign countries and to authorize others to make such uses of the work. These rights will become the property of Health Science Journal of Indonesia from the date of acceptance of the article for publication and extend for the life of the copyright. I understand that Health Science Journal of Indonesia, as copyright owner, has authority to grant permission to reproduce the article.
dc.sourceHealth Science Journal of Indonesia; Vol 8, No 1 (2017); 7-11en-US
dc.sourceHealth Science Journal of Indonesia; Vol 8, No 1 (2017); 7-11id-ID
dc.subjecten-US
dc.subjecten-US
dc.subjecten-US
dc.titleDetection of Genotype D8 Measles Virus in Indonesia in 2014en-US
dc.typePeer-reviewed Articleen-US


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