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dc.contributorNational Institute of Health Research and Developmenten-US
dc.creatorWidiastuti, Dyah; Biologist, Animal Borne Disease Control Unit Banjarnegara, National Institute of Health Research and Development, Ministry of Health of Republic of Indonesia, Indonesia
dc.creatorIkawati, Bina
dc.creatorMartini, Martini
dc.creatorWijayanti, Nastiti
dc.date2017-12-31
dc.date.accessioned2019-12-17T00:37:20Z
dc.date.available2019-12-17T00:37:20Z
dc.identifierhttp://ejournal.litbang.depkes.go.id/index.php/HSJI/article/view/6854
dc.identifier10.22435/hsji.v8i2.6854.74-80
dc.identifier.urihttp://r2kn.litbang.kemkes.go.id:8080/handle/123456789/81045
dc.descriptionLatar Belakang: Resistensi terhadap insektisida terutama terjadi karena adanya perubahan pada enzim metabolik serangga. Enzim metabolik yang sering berperan dalam kejadian resistensi antara lain adalah esterase dan monooksigenase. Metode: Uji kerentanan dan uji biokimia untuk mendeteksi resistensi terhadap malation dan cypermetrin dilakukan pada Aedes aegypti dari Wonosobo (daerah endemis baru infeksi Dengue di dataran tinggi). Uji coba yang dilakukan pada generasi F1 nyamuk Ae.aegypti yang tertangkap di lapangan bertujuan untuk mengetahui mekanisme resistensi berdasarkan aktivitas dua enzim detoksifikasi yaitu esterase dan monooksigenase. Wawancara dengan menggunakan kuesioner terstruktur dilakukan untuk mengetahui penggunaan insektisida oleh masyarakat dan pemerintah daerah. Hasil: Uji kerentanan menunjukkan mortalitas sebesar 23,4% setelah terpapar malathion 0,8% dan 46,7% setelah terpapar cypermethrin 0,05%. Hasil uji biokimia menunjukkan bahwa aktivitas esterase dan monooksigenase cenderung meningkat pada Ae.aegypti di Wonosobo. Wawancara dan kuesioner menyimpulkan bahwa cypermetrin adalah satu-satunya golongan insektisida yang digunakan dalam program pengendalian vektor oleh Dinas Kesehatan Wonosobo dan merupakan tipe insektisida yang paling sering digunakan di rumah tangga oleh masyarakat Wonosobo untuk mengendalikan populasi Ae.aegypti. Kesimpulan: Ditemukan nyamuk Ae. aegypti yang mengalami peningkatan aktivitas enzim esterase dan monooksigenase pada populasi Ae. aegypti di Kabupaten Wonosobo. Hal ini selaras dengan status resistensi populasi nyamuk tersebut yang resisten terhadap Malation dan Cypermetrin. Kata kunci: Ae.aegypti Wonosobo, biokimia, paparan insektisida, resistensi Abstract Background: Resistance to insecticides mainly occurs due to changes in insect metabolic enzyme. A metabolic enzyme which was often involved in insecticide resistance is esterase and monooxygenase. Methods: Susceptibility test and biochemical assay to detect malathion and cypermethrin resistance were conducted on Aedes aegypti from Wonosobo (new highland Dengue endemic area). The test was performed on F1 generation of Ae.aegypti field caught mosquitoes which aimed to determine the resistance mechanisms regarding two detoxifying enzymes i.e. esterase and monooxygenase. Interview using structured questionnaires was conducted to investigate the usage of insecticide by the society and local government. Results: Susceptibility test showed 23.4 and 46.7% mortalities after exposure to 0.8% malathion and 0.05% cypermethrin. The biochemical assay result suggested that esterase, and monooxygenase activity tend to increase in Ae.aegypti in Wonosobo. Interview and questionnaires conclude that synthetic pyrethroid was the only insecticide type used in vector control program by Wonosobo Health Office and was the most frequent insecticide type to be used in household by Wonosobo society to control Ae.aegypti population.Conclusion: Aedes aegypti with increased esterase and monooxygenase activity were found in Wonosobo. This result was in line with the resistance status of Ae. aegypti population in Wonosobo which resistant to Malathion and Cypermethrin. Keywords: Ae.aegypti Wonosobo, biochemical, insecticide exposure, resistanceen-US
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dc.languageen
dc.publisherBadan Penelitian dan Pengembangan Kesehatanen-US
dc.rightsThe Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Health Science Journal of Indonesia and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.
dc.rightsUnder the terms of Indonesian Goverment Copyright Law, Health Science Journal of Indonesia must formally obtain transfer of copyright before an article can be published. Health Science Journal of Indonesia grants to the author (and to all coauthors) the right to present orally in any forum all or part of the work. It is also stipulated that all graphics including figures, tables and charts may be republished with permission from Health Science Journal of Indonesia. In consideration of the fact that Health Science Journal of Indonesia undertakes to publish my article: I affirm that the material has not been previously published and that I (and my coauthors) own and have not transferred elsewhere any rights to the article.I affirm that I (and my coauthors) have obtained written permission to use any previously copyrighted material included in the article and that such documentation will be forwarded to Health Science Journal of Indonesia simultaneously with the article. I further affirm that I (and my coauthors) have stated any possible conflicts of interest within the article.I (and my coauthors) hereby assign and transfer to Health Science Journal of Indonesia all rights of copyright ownership and permission to the article, including without limitation or restriction, all rights of reproduction, derivation, distribution, sale, reuse, and display of the of the work, in whole or in part, including recompilation and stand-alone publication, in any and all forms of media now or hereafter known, including all electronic and digital media, as protected by the laws of the Republic of Indonesia and foreign countries and to authorize others to make such uses of the work. These rights will become the property of Health Science Journal of Indonesia from the date of acceptance of the article for publication and extend for the life of the copyright. I understand that Health Science Journal of Indonesia, as copyright owner, has authority to grant permission to reproduce the article.
dc.sourceHealth Science Journal of Indonesia; Vol 8, No 2 (2017); 74-80en-US
dc.sourceHealth Science Journal of Indonesia; Vol 8, No 2 (2017); 74-80id-ID
dc.subjectpublic health-entomologyen-US
dc.subjectAe.aegypti Wonosobo, biochemical, insecticide exposure, resistanceen-US
dc.subjectvector ecologyen-US
dc.titleBiochemical characterization of insecticide resistance and exposure in Aedes aegypti population from Wonosobo (a new highland Dengue endemic area), Central Java, Indonesiaen-US
dc.typePeer-reviewed Articleen-US


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