Natural steroids can be used for pharmaceutical production, especially served as starting material for their partial synthesis of sex hormones, including the oral contraceptives. In this research, steroid was obtained as byproducts found during isolation and identification of cytotoxic compounds from Stelecbocarpus burabol Hook f. & Thomson stem bark. The steroid mixture appears as white powder, consist of 3 steroid components: .6.s-ergostenol, stigmasterol, and ~sitosterol. Structure elucidation has been done using GC-MS and lH-NMR speetroscopic. The result was compared with literature standard. Key words: steroid mixture, stem bark, Stelecbocarpus burabol.
