An efficacy assessment of insecticide impregnated bed nets PermaNet “Vestegaard –Frandsen” effectiveness was conducted in some villages of the malaria endemic areas around theBukit Manoreh (Magelang, Purworejo and Kulonprogo Regency). The nsecticide impregnatedbed nets PermaNet “Vestegard –Frandsen” were distributed on May 2003 and to be evaluated onFebruary 2004. The efficacy assessment was conducted using bioassay method according toWHO standard against wild female Anopheles aconitus from each regency showed thatinsecticide impregnated bed nets PermaNet” Vestegaard –Frandsen” have been used for aboutone year was not effective for controlling mosquitoes (The mortality of An. aconitus < 70%).Bioassay test of new bed net PermaNet “Vestegard –Frandsen” using An. aconitus fromlaboratory koloni revealed 90% mortality