A study was conducted to determine 1). The optimum of pH coconut water for proliferation cell and spore B. thuringiensis H-14 local strain 2). The LC50 (Lethal Concentration 50 %) and LC95 Ae. aegypti dan An. aconitus mosquito larvae from various kinds of coconut water for 24 hours testing. 3). The measure contents of the nutrition ( carbohydrate, fat, protein and reduced glucose) coconut water for proliferation B. thuringiensis H-14 local strain.. This study was using 6-8 months age coconuton average weight around 1 kg that contained water approximately 400-500 ml/coconut. Those coconuts were taken from Kunir Rejo village, Butuh regency, Purworejo district. Fifteen out of 20 coconuts were used to culture living cells and spore of B. thuringiensis H-14 local strain on Institute of Vector and reservoir Control Research and Development Salatiga laboratory and the rests of them were used to analyze the contain of coconut water (it was done in the Institute of Health Laboratory Semarang). The results showed pH 7 as the optimum pH for proliferation cells and spore of B. thuringiensis H-14 local strain at coconut water media with LC50 dan LC95 against Ae. aegypti and An. aconitus larvae for 24 hours of exposure were LC50 = 21.77 ppm and LC95 = 64,00 ppm ; LC50 = 0.0004 ppm and LC95 = 0.0010 ppm. respectively. It has 1.92%, carbohydrate, 0.01% fat, 0.06% protein and reduced glucose 1,87 %. Coconut water can be used as an alternative local media to culture B. thuringiensis H-14 local strain.