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dc.creatorWidyastuti, Umi; Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit, Salatiga
dc.date2013-12-10
dc.date.accessioned2019-12-17T10:44:51Z
dc.date.available2019-12-17T10:44:51Z
dc.identifierhttp://ejournal.litbang.depkes.go.id/index.php/vk/article/view/3334
dc.identifier10.22435/vektora.v5i1 Jun.3334.18-27
dc.identifier.urihttp://r2kn.litbang.kemkes.go.id:8080/handle/123456789/83605
dc.descriptionAbstrakMalaria masih merupakan masalah kesehatan di Kabupaten Magelang, khususnya di Kecamatan Borobudur. Annual Parasite Incidence (API) dua tahun terakhir sebesar 0,19 pada tahun 2004 dan meningkat 0,34 pada tahun 2005, menunjukkan status Low Case Incidence (LCI). Kasus malaria di daerah tersebut sehubungan dengan  adanya  beberapa  spesies  nyamuk  Anopheles  yang  potensial  sebagai  vektor  malaria.  Kompetensi vektorial  nyamuk  Anopheles  di  Kecamatan  Borobudur  belum  banyak  dilaporkan,  khususnya  halam  hal kerentanannya terhadap Plasmodium dan sifat antropofilik (kesukaan menghisap darah manusia). Berbagai spesies seperti Anopheles aconitus, An maculatus, dan An. balabacensis merupakan tersangka vektor malaria di daerah tersebut. Tujuan penelitian ini adalah: a). mendeteksi antigen protein circum sporozoit P. falciparum atau P. vivax pada nyamuk Anopheles sp dengan teknik Enzyme Linked Immunosarbant Assay (Elisa) dan b).  mengidentifikasi  pakan  darah  manusia  pada  nyamuk  Anopheles  spp  dengan  teknik  Elisa.  Penelitian dilaksanakan pada bulan Januari-Mei 2006 di 2 desa HCI yaitu di Giripurno dan Giritengah, Kecamatan Borobudur. Penelitian dilakukan dengan menangkap nyamuk yang istirahat di dalam dan luar rumah pada malam hari (18.00-12.00) dan pagi hari (06.00-08.00) sesuai dengan metode WHO, 2003. Nyamuk Anopheles spp dipisahkan berdasarkan spesies untuk dihitung kepadatannya. Selanjutnya dilakukan pembedahan ovarium untuk  mengetahui  paritasnya  (parous  atau  nulliparous). Anopheles  spp  parous  (4  spesies  yaitu Anopheles aconitus, An maculatus, An. balabacensis dan An. barbirostris) diperiksa kondisi abdomennya untuk kepentingan pengujian dengan ELISA. Keempat spesies nyamuk parous (semua kondisi abdomen yaitu unfed, blood fed, half gravid dan gravid) diambil bagian dada-kepala untuk kepentingan Elisa sporozoit). Nyamuk parous dengan kondisi blood fed dan half gravid diambil bagian abdomennya, dipencet di atas kertas Whatman dan digunakan  untuk  ELISA  pakan  darah.  Hasil  penelitian  menunjukkan  bahwa An.  aconitus  rentan  terhadap P. falciparum dengan angka sporozoit sebesar 0,07 % di Giripurno dan sporozoit P. vivax tidak ditemukan, sedangkan di Giritengah, An. balabacensis rentan terhadap P. falciparum dengan angka sporozoit 4,17 % dan sporozoit P. vivax tidak ditemukan. Proporsi An. aconitus menghisap darah manusia (HBI) sebesar 10,34 % di Giripurno dan 5,97 % di Giritengah. An balabacensis dan An. barbirostris menunjukkan HBI sebesar 37,50 % dan 5,88% di Giritengah. Angka paritas dan kepadatan An. aconitus di Giripurno relatif lebih tinggi dibanding di Giritengah, sebaliknya An. balabacensis lebih tinggi di Giritengah dibanding di Giripurno.Kata kunci: malaria, Elisa sporozoit, Elisa pakan darah.AbstractMalaria is still a health problem in Magelang Regency, especially in the Borobudur Subdistrict. The Annual Parasite Incidence (API) in the last two years were 0.19 in 2004 and increased 0.34 in 2005, were considered as malarious areas with Low Case Incidence (LCI). The increase of malaria cases in Borobudur Subdistrict is related to the presence of Anopheline mosquitoes which serve as potential vector. The vectorial competence of Anopheline mosquitoes in Borobudur Subdistrict has not been reported yet. Several species such as Anopheles aconitus, An. maculatus, An. barbirostris and An. balabacensis are suspected as potential malaria vectors in this area. The objective of this study was to determine the Anophelene mosquitoes susceptibility to Plasmodia and its anthropophilic characteristic. The susceptibility of mosquito to Plasmodia was measured by detection of sporozoite protein antigen (Circum Sporozoite Protein/ CSP) of P. falciparum or P. vivax on the head-thorax of all parous mosquitoes. The anthropophilic characteristic was measured by detection of human blood on the abdomen of blood fed and half gravid mosquitoes. Both of these were done by Elisa technique. A study was  conducted  from  Januari  to  May  2001  in  two  HCI  villages  i.e:  Giripurno  and  Giritengah,  Borobudur Subdistrict. The Anopheline mosquitoes were collected using the resting mosquito collection technique both indoors and outdoors, at night (18.00-24.00) as well as in the morning (06.00-08.00) according to the WHO guideline. The density of Anophelenes (4 species) was calculated and its parity were determined by microscopic ovary dissection. The result showed that An. aconitus in Giripurno was susceptible to P. falciparum with a sporozoite rate of 0.07 %. However, CSP antigen of P. falciparum was not detected in the head-thorax of mosquitoes from Giritengah. An. balabacensis in Giritengah was susceptible to P. falciparum with a sporozoite rate of 4.17 %. However, CSP antigen of P. falciparum was not detected in the head-thorax of mosquitoes from Giripurno. CSP antigen of P. vivax was also negative in the head-thorax of mosquitoes both from Giripurno and Giritengah villages. The proportion of An. aconitus fed on human (Human Blood Index / HBI) was 10.34 % in Giripurno and 5.97 % in Giritengah. HBI of An. balabacensis and An. barbirostris were 37.50 % and 5.88 % respectively in Giritengah. The parity rate of An. aconitus and its density was found higher in Giripurno than in Giritengah. In contrast, the parity rate and density of An. balabacensis was found higher in Giritengah than in Giripurno.Keywords: malaria, sporozoite Elisa, blood meals identificationid-ID
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dc.publisherBalai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (B2P2VRP) Salatigaen-US
dc.rightsThe Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to VEKTORA and Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (Institute of Vector and Reservoir Control Research and Development) as publisher of the journal. Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from VEKTORA (Journal of Vector Borne and Reservoir Diseases) and Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (Institute of Vector and Reservoir Control Research and Development). VEKTORA (Journal of Vector Borne and Reservoir Diseases) and Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (Institute of Vector and Reservoir Control Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.
dc.sourceVektora : Jurnal Vektor dan Reservoir Penyakit; Vol 5, No 1 Jun (2013); 18-27en-US
dc.sourceVektora; Vol 5, No 1 Jun (2013); 18-27id-ID
dc.subjectid-ID
dc.subjectid-ID
dc.subjectmalaria, sporozoite Elisa, blood meals identificationid-ID
dc.titleINKRIMINASI VEKTOR MALARIA DAN IDENTIFIKASI PAKAN DARAH PADA NYAMUK AnopHELEs SPP DI KECAMATAN BOROBUDUR, KABUPATEN MAGELANGid-ID
dc.typeen-US


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