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dc.creatorSamarang, Samarang; Balai Litbang P2B2 Donggala, Badan Penelitan dan Pengembangan Kesehatan, Kementerian Kesehatan RI
dc.date2017-08-25
dc.date.accessioned2019-12-17T10:47:09Z
dc.date.available2019-12-17T10:47:09Z
dc.identifierhttp://ejournal.litbang.kemkes.go.id/index.php/vektorp/article/view/7496
dc.identifier10.22435/vektorp.v7i1.7496.30-35
dc.identifier.urihttp://r2kn.litbang.kemkes.go.id:8080/handle/123456789/83795
dc.descriptionFasciolosis is a parasitic disease that caused by liver flukewormfromTrematode class and Fasciola genus with two important species, Fasciola hepatica and Fasciola gigantica (F.gigantica). Early diagnosis of fasciolosis can be detected by ELISA method to recognize antibody used antigen capture or known as coproantigen. Concentrations test on protein antigen (Ag) exretory-secretory (ES) of F. gigantica was conducted in September 2010. Antigen ES used in this test, is an AgES which had been produced in previous research. ES antigens can be used as a material for producing antibodies and as an ingredient in the ELISA test. The purpose of this studywas to determine the protein concentration of bovine AgESFg taken fromlocal cattle in Sigi district. The studywas an observational study with a cross-sectional study design. Samples of infected cattle liver were randomly taken from the Sigi Biromaru abattoir (slaughterhouse). ESFg antigens produced by collecting worms F. gigantica from Sigi local cattle and then isolated and incubated using RPMI culture medium for four hours at 370C. ESFg antigens characterized using Bradford method. Characterization produced AgES from three times collections of worms F. gigantica with obtained concentration were 0430, 0459, 0699 mg/ml within an average of 0.18 mg/worm. Therefore, it can be concluded that the protein concentration were 0,430- 0,699 mg/ml AgESFg taken from local cattle in Sigi district can be used as a material for producing antibodiesand asaningredient in the ELISA test.en-US
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dc.languageen
dc.publisherBalai Penelitian dan Pengembangan Pengendalian Penyakit Bersumber Binatang Donggalaen-US
dc.rightsPublishing your paper with Jurnal Vektor Penyakit (JVP) means that the author or authors transfer the copyright to JVP. JVP granted an exclusive reuse license by the author(s), but the author(s) are able to put the paper onto a website, distribute it to colleagues, give it to students, use it in your thesis etc, even commercially. JVP journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets others remix, transform, and build upon the material for any purpose, even commercially. JVP journal Open Access articles are distributed under this Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA). Articles can be read and shared for All purposes under the following conditions: BY: You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. SA: If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.  Download Copyright Transfer Form
dc.sourceJurnal Vektor Penyakit; Vol 7, No 1 (2013); 30-35id-ID
dc.sourceJurnal Vektor Penyakit; Vol 7, No 1 (2013); 30-35en-US
dc.subjectFasciolosis; Excretory-secretory antigen; Fasciola giganticaen-US
dc.titleKonsentrasi Protein Antigen Ekskretori-Sekretori Fasciola Gigantica Asal Sapi Lokal Kabupaten Sigi, Sulawesi Tengahen-US
dc.typePeer-reviewed Articleen-US


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