Diseases transmitted by mosquito such as maluria, dengue hemorrhagic fever and filariasis are still public health problems that cause outbreak and death in Indonesia. Mosquitoes such as Aedes sp, Culex sp, Anopheles sp and Mansonia sp are the main disease vector. A study that aimed to know Anopheles sp fauna and competency was conducted in Santu'un village, Muara Uya sub-district, Tabalong district. Mosquitoes were collected by using human-biting catch and cattle pen resting catch methods with aspiration. ELIZA test was used to find sporozoite in Anopheles sp. An. umbrosus was found to be endophagic whereas An. nigerimus and An.kochi was exophagic. The peak biting periods of An. umbrosus and An. nigerimus were occur belween 8 and 9 pm with biting rates of 0.5 bites/man/night. The human biting activity of An. kochi was occur between 6 and 7 pm with biting rates of 1.5 bites/man/night. The Anopheles sp breeding places were in muddy puddles, tires track, step track, and water container. The water temperature was 25C with pH of 5.6 and salinity of 0. No mosquitoes were found positive as vector of malaria.