Anemia allevation program by iron-folat tablet supplementation has not shown satisfaction results. Food based approach should be done to provide more sustainable intake. The purpose of this study is to determine the nutritional quality of crackers product millet (Pennisetum sp) substituted in various concentration and its capability to increase hemoglobin levels on wistar rats. This in an experimental study with complete randomised design one factor. The concentration substituted divided into 3 treatment, of 0%, 10% and 20% concentration with 7 replication. The dependent variabel were nutritional quality (water content, ash, protein, fat, fiber, carbohydrate, iron (content), and hemoglobin concentration). The wistar rats had been fed during for 14 days. Data analysis were conducted using ANOVA and t-test. Millet substitution resulted in the decrease of water, crude fiber, carbohydrate with (7.24% to 6.54%, p=0,00), (15.49% to 14.54%, p=0,00), (56.61% to 52.58%, p=0,00) respectivelly. The substitution increased of ash, protein, fat and iron (1.96% to 2.37%, p=0,00), (14.42% to 14.67%, p=0,00), (19.76% to 23.83, p=0,00), (66.80 ppm to 82.83 ppm, p=0,371). Respectivelly. The hemoglobin increase on 0% concentration were 0,97 g/dl; on 10% were: 2,92 g/dl and on 20% were: 3,39 g/dl. It was concluded that 20% millet substitution in crackers product given to the wistar rats 14 days significantly increased hemoglobin concentration by 3,39 g/dl.