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dc.contributoren-US
dc.creatorWibowo, Agus
dc.date2018-12-25
dc.date.accessioned2019-12-17T10:49:05Z
dc.date.available2019-12-17T10:49:05Z
dc.identifierhttp://ejournal.litbang.depkes.go.id/index.php/mgmi/article/view/9551
dc.identifier10.22435/mgmi.v4i2.9551.137-148
dc.identifier.urihttp://r2kn.litbang.kemkes.go.id:8080/handle/123456789/83926
dc.descriptionHiperthyroid is execcesive thyroid function which increased thyroid hormone that suppressed TSH. Major etiologies of hyperthyroidism (60%-80%) caused by Graves disease. Graves disease occurs in up to 2% women 20-40 years old. Some of the genes implicated in this pathogenesis may encode thyroid stimulating hormones receptors (TSHR). The TSHR gene is located on chromosome 14q31, an area in which a Graves disease susceptibility locus (GD-1) has been mapped. The GD-1 locus is specifically linked to Graves’ disease. TSHR polymorphisms, resulting in amino acid substitutions, have been identified. Two of these are located in the extracellular domain of the receptor (Asp36His and Pro52Thr), and one is located in the intracellular domain (Asp727Glu). A germline mutation of TSHR codon 727 has been reported to be associated with Graves disease. The aim of this research is to analyze the polymorphism of codon D727E on hiperthyroid patient. Sixteen respondents with suspect hyperthyroidism were chosen. The blood were collected for TSH, FreeT4, Thyroglobulin Antibody and Thyroidperoxidase Antibody and DNA isolation. The polymorphic region of the target genes (gene TSHR codon 727) were amplified by polymerase chain reaction (PCR). PCR-Sequencing was used for polymorphism on codon D727E confirmation. The result showed there is no Polymorphism in gen TSHR codon D727E. The codon D727E variant of TSHR gene is not associated with Hyperthyroid in Sukoharjo population. The possibility that mutation may occur on another genes need to be further analyzed.en-US
dc.formatapplication/pdf
dc.languageen
dc.publisherBalai Penelitian dan Pengembangan Gangguan Akibat Kekurangan Iodiumen-US
dc.rightsMedia Gizi Mikro Indoesia (MGMI) Journal provides immediate open access to its content on the principle that making research freely available to the public supporting a greater global exchange of knowledge.All articles published Open Access will be immediately and permanently free for everyone to read and download.  We are continuously working with our author communities to select the best choice of license options, currently being defined for this journal as follows:• Creative Commons Attribution (CC-BY-SA)The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to MGMI Journal and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.  
dc.sourceMedia Gizi Mikro Indonesia; Vol 4, No 2 (2013): Edisi Juni 2013; 137-148id-ID
dc.sourceMedia Gizi Mikro Indonesia; Vol 4, No 2 (2013): Edisi Juni 2013; 137-148en-US
dc.subjectHipertiroid, penyakit Graves, TSHR kodon D727E, PCRen-US
dc.titleDETEKSI POLIMORFISME GEN TSHR CODON D727E PADA WANITA TERDUGA HIPERTIROID DI KABUPATEN SUKOHARJOen-US
dc.typeen-US


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