Abstract

AbstractAedes aegypti is a major vector of Dengue, a deadly disease causing death of millions of people in developing countries both in urban and rural populations. Ae. aegypti control using chemical insecticide was always done and lead to a widespread insecticide resistance. So, mosquito biological control was needed to replace the usage of chemical insecticide. An experimental study using completely Randomized Design was conducted during March-April 2016 at Balai Litbang P2B2 Banjarnegara. A biolarvicide formula was made from Beauveria bassiana secondary metabolite which propagated by The Bacteriology Laboratory of Balai Litbang P2B2 Banjarnegara. In the experimental method, four concentrations of biolarvacide formula (2%, 4%, 8% and16%) were exposed to 3rd instar of Ae.aegypti larvae for eight days. The result showed that exposure of biolarvacide formula causing larval mortality which started on the 1st day exposure. This result showed that secondary metabolite of B. bassiana contains some larvacidal compounds.Keywords: Beauveria bassiana, secondary metabolite, Aedes aegyptiAbstrakAedes aegypti merupakan vektor utama Dengue, penyakit yang menyebabkan kematian jutaan orang di negara-negara berkembang, baik pada populasi perkotaan dan pedesaan. Pengendalian Ae. aegypti menggunakan insektisida kimia selalu dilakukan dan menyebabkan resistensi insektisida secara luas. Oleh karena itu, pengendalian nyamuk secara biologis diperlukan untuk menggantikan penggunaan insektisida kimia. Penelitian eksperimental dengan disain rancangan acak lengkap dilaksanakan pada bulan Maret-April 2016 di Balai Litbang P2B2 Banjarnegara. Formula insektisida hayati dibuat dari metabolit sekunder jamur Beauveria bassiana. Metode eksperimen, menggunakan empat konsentrasi formula larvasida hayati (2%, 4%, 8% dan 16%) dipaparkan pada larva Ae.aegypti selama delapan hari. Hasil penelitian menunjukkan bahwa formula larvasida hayati menyebabkan kematian larva instar III Ae.aegypti. Kematian larva uji dimulai pada hari pertama. Hasil tersebut menunjukkan bahwa dalam metabolit sekunder jamur B. bassiana terdapat senyawa yang memiliki efek larvasida.Kata Kunci: Beauveria bassiana, metabolit sekunder, Aedes aegypti